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    Abcam ELISA Questionnaire  
   
NAME :  
TELEPHONE NO :  
EMAIL :    
1. Abcam catalogue no :
2. a) Abcam order number :
2. b) Abcam product lot number :
3. Description of the problem :
(high background, Low or week signal , Problem with standard curve etc)
4. Antibody storage conditions :
(temperature/reconstitution etc)
5. a) Sample (Species) :
5. b) Type of sample :
(Cell extract/Nuclear extract/Purified protein/Recombinant protein etc)
5. c) Type of Elisa :
( Direct Elisa , Indirect Elisa, Sandwitch Elisa etc)
6. a) Sample preparation:Coating well :
Lysis buffer
Concentration of coating materials
Incubation times
6. b) Amount of protein loaded :
Protein loaded ug/lane or cells/lane
7. Transfer or blocking conditions :
Type of membrane
Protein transfer verified
Blocking agent and concentration
Blocking time
Blocking temperature
8. Primary antibody
(If more than one was used, describe in “additional notes”) :
Concentration or dilution
Diluent buffer
Incubation time
Incubation temperature
Washing: Buffer Used
Washing: Number of washes
9. Secondary antibody :
Species
Reacts against
Concentration or dilution
Diluent buffer
Incubation time
Incubation temperature
Fluorochrome or enzyme conjugate
Washing: Buffer Used
Washing: Number of washes
10. Detection method :
(Substrate /Diluent.)
11. Positive and negative controls used :
Positive control
Negative control
12. a) How many times you have run this staining? :
12. b) Do you obtain the same results every time? :
12. c) What steps have you altered to try and optimize the use of this antibody? :
Document attachment :
Attaching images of your blot is strongly recommended and can greatly speed up our investigation of your problem. Make single zip file and attach.
 
 
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