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    Abcam IHC Questionnaire  
   
NAME :  
TELEPHONE NO :  
EMAIL :    
1. Abcam product code :
2. Abcam order reference number or product batch number :
3. Description of the problem :
(high background, Low or week signal , Problem with standard curve etc)
4. Antibody storage conditions :
(temperature/reconstitution etc)
5. Sample :
(Fresh frozen sections, perfusion fixed frozen sections, PFA/formalin fixed paraffin embedded sections, cells in culture, other):
6. Sample preparation :
7. Blocking :
Yes/No
Blocking agent and concentration
Blocking time
Blocking temperature
Endogenous peroxidases blocked? Yes/No
Endogenous biotins blocked? Yes/No
8. Fixation Step :
Fixation: Yes/No
Fixative agent and concentration
Fixation time
Fixation temperature
9. Antigen retrieval method :
10. Permeabilization :
Yes/No
Permeabilization agent and concentration
Permeabilization time
Permeabilization temperature
11. Primary antibody (If more than one was used, describe in “additional notes”) :
Concentration or dilution
Diluent buffer
Incubation time
Incubation temperature
12. Secondary antibody :
Species
Reacts against
Concentration or dilution
Diluent buffer
Incubation time
Incubation temperature
Fluorochrome or enzyme conjugate
13. a) Washing After primary antibody :
Buffer
Number of washes
13. b) Washing After secondary antibody :
Buffer
Number of washes
14. Detection method :
(Substrate /Diluent.)
15. Positive and negative controls used (please specify):
15. a) How many times you have run this staining? :
15. b) Do you obtain the same results every time? :
15. c) What steps have you altered to try and optimize the use of this antibody? :
Document attachment :
Attaching images of your blot is strongly recommended and can greatly speed up our investigation of your problem. Make single zip file and attach.
 
 
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