ELISA Kit for Human Interferon Gamma Induced Protein 10kDa (IP10), 96 Tests

The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IP10 in human serum,plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Item Name

Interferon Gamma Induced Protein 10kDa (IP10)

Alternative Names

CXCL10; C7; IFI10; INP10; SCYB10; Crg2; GIP10; Mob1; Chemokine C-X-C Motif Ligand 10; Small Inducible Cytokine Subfamily B(Cys-X-Cys) Member 10.

Organism Species
Homo sapiens (Human) 
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3h
Detection Range
15.6-1,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 5.5pg/mL.

Specificity
This assay has high sensitivity and excellent specificity for detection of Interferon Gamma Induced Protein 10kDa (IP10).
No significant cross-reactivity or interference between Interferon Gamma Induced Protein 10kDa (IP10) and analogues was observed.

Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Gamma Induced Protein 10kDa (IP10) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Gamma Induced Protein 10kDa (IP10) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Shipping & Storage

Shipped at 4°C. 

For unused kit: The whole kit could be stored at -20oC in shelf life, while up to one month at 4oC. For experiment convenience, reagents could also be stored seperately, Standard, Detection Reagent A, Detection Reagent B and 96-well strip plate should be stored at -20oC while the others could be at 4 oC.

Citations of Primary Manufacturer

Magazine Citations
20 Melatonin inhibits type 1 interferon signaling of toll-like receptor 4 via heme oxygenase-1 induction in hepatic ischemia/reperfusion. PubMed: 22288937
BMC Pregnancy and Childbirth Maternal circulating leukocytes display early chemotactic responsiveness during late gestation PubMed: PMC3561147
Rheumatology International Serum levels of selected chemokines in systemic lupus erythematosus patients Pubmed: 22461186
PLOS ONE The Immunosuppressant Protosappanin A Diminished Recipient T Cell Migration into Allograft via Inhibition of IP-10 in Rat Heart Transplant Pubmed:24798458
Front Pharmacol The G-Protein-Coupled Bile Acid Receptor Gpbar1 (TGR5) Inhibits Gastric Inflammation Through Antagonizing NF-κB Signaling Pathway PubMed: 26696888
Cytokine Hypoxia/ischemia promotes CXCL10 expression in cardiac microvascular endothelial cells by NFkB activation Pubmed:26891076
Journal of Experimental & Clinical Cancer Research LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4. pubmed:28641579
Journal of Geriatric Oncology Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors Pubmed: 30952517
Hepatology Integrated Omics Reveals Tollip as an Aggravator and Therapeutic Target for Hepatic Ischemia‐Reperfusion Injury in Mice Pubmed: 31077413
Molecular Medicine Reports IL‑17A promotes CXCR2‑dependent angiogenesis in a mouse model of liver cancer Pubmed: 31173199
JOURNAL OF MOLECULAR NEUROSCIENCE Inflammatory Responses of Astrocytes Are Independent from Lipocalin 2 Pubmed: 32959226