Next Gen DNA Library Kit

Next Gen DNA Library Kit

Code: 53216

The Next Gen DNA Library Kit is designed to prepare Next generation sequencing (NGS) libraries from double-stranded input DNA for use with Illumina® sequencing platforms. Libraries can be generated from as little as 10 pg of high-quality genomic DNA, ChIP DNA, FFPE DNA or cell-free DNA (cfDNA), which is 500-fold more sensitive than other library preparation kits. Additionally, libraries can be prepared from 100 ng DNA if preparing PCR-free libraries.

Next Gen DNA Library Kit is used in combination with the Next Gen Indexing Kit, which contains 16 unique index adapters that may be used individually, or in combination to multiplex samples together during cluster generation for co-sequencing on the same Illumina flow cell. The advantage of the Next Gen DNA Library preparation is the inclusion of molecular identifiers (MIDs) to enable accurate removal of PCR duplicates from single read sequencing. This helps to increase the number of unique alignments for more accurate data sets.

Please note that the Next Gen DNA Library and Next Gen Indexing Kits are shipped on dry ice and contains reagents with multiple storage temperatures inside. All components can be stored at -20°C prior to first use, then we recommend storing each component at the temperatures indicated below. All reagents are guaranteed stable for 6 months from date of receipt when stored properly. Each kit includes the following components:

Reagents for Next Gen DNA Library Kit (Cat. No. 53216)

  • Buffer W1; Store at -20°C

  • Enzyme W2; Store at -20°C

  • Buffer G1; Store at -20°C

  • Reagent G2; Store at -20°C

  • Enzyme G3; Store at -20°C

  • Enzyme G4; Store at -20°C

  • Buffer Y1; Store at -20°C

  • Enzyme Y3 Store at -20°C

  • Buffer B1; Store at -20°C

  • Reagent B3; Store at -20°C

  • Enzyme B4; Store at -20°C

  • Enzyme B5; Store at -20°C

  • Enzymbe B6; Store at -20°C

  • Reagent R2; Store at -20°C

  • Buffer R3; Store at -20°C

  • Enzyme R4; Store at -20°C

  • Alu 115; Store at -20°C

  • Alu 247; Store at -20°C

  • PEG Solution; Store at RT

  • Low EDTA TE; Store at RT

Reagents for Next Gen Indexing Kit (Cat. No. 53264)

  • Reagent Y2 Index 1; Store at -20°C

  • Reagent Y2 Index 2; Store at -20°C

  • Reagent Y2 Index 3; Store at -20°C

  • Reagent Y2 Index 4; Store at -20°C

  • Reagent Y2 Index 5; Store at -20°C

  • Reagent Y2 Index 6; Store at -20°C

  • Reagent Y2 Index 7; Store at -20°C

  • Reagent Y2 Index 8; Store at -20°C

  • Reagent Y2 Index 9; Store at -20°C

  • Reagent Y2 Index 12; Store at -20°C

  • Reagent Y2 Index 13; Store at -20°C

  • Reagent Y2 Index 14; Store at -20°C

  • Reagent Y2 Index 15; Store at -20°C

  • Reagent Y2 Index 16; Store at -20°C

  • Reagent Y2 Index 18; Store at -20°C

  • Reagent Y2 Index 19; Store at -20°C

  • Reagent R1; Store at -20°C

  • Reagent B2 MID; Store at -20°C.

Next Gen DNA Library Kit Highlights:

  • NGS libraries for use with Illumina platforms (MiSeq, HiSeq 2500, NextSeq 500)

  • Works with double-stranded input DNA including:  genomic DNA, ChIP DNA, FFPE DNA and cell-free DNA (cfDNA)

  • Use as little as 10 pg DNA (500-fold less input material required than other kits)

  • Prepare PCR-free libraries with only 100 ng DNA

  • Indexing kit contains 16 unique indices that can be multiplexed together within the same sequencing flow cell

  • Compatible with Active Motif's ChIP-IT High Sensitivity® Kit for a complete ChIP-seq workflow.