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    Abcam WB Questionnaire  
   
NAME :  
TELEPHONE NO :  
EMAIL :    
1. Abcam catalogue no :
2. a) Abcam order number :
2. b) Abcam product lot number :
3. Description of the problem :
(high background, wrong band size, more bands, no band etc)
4. Antibody storage conditions :
(temperature/reconstitution etc)
5. a) Sample (Species) :
5. b) Type of sample :
(Cell extract/Nuclear extract/Purified protein/Recombinant protein etc)
6. a) Sample preparation :
Lysis buffer
Protease inhibitors
Phosphatase inhibitors
Reducing agent
Boiling for ≥5 min?
6. b) Amount of protein loaded :
Protein loaded ug/lane or cells/lane
7. a) Electrophoresis/Gel conditions :
Reducing or Non Reducing gel
Percentage of gel
Volts applied
Time applied
7. b) Transfer or blocking conditions :
Type of membrane
Protein transfer verified
Blocking agent and concentration
Blocking time
Blocking temperature
8. Primary antibody
(If more than one was used, describe in “additional notes”) :
Concentration or dilution
Diluent buffer
Incubation time
Incubation temperature
Washing: Buffer Used
Washing: Number of washes
9. Secondary antibody :
Species
Reacts against
Concentration or dilution
Diluent buffer
Incubation time
Incubation temperature
Fluorochrome or enzyme conjugate
Washing: Buffer Used
Washing: Number of washes
10. Detection method :
(ECL, ECL plus, etc.)
11. Positive and negative controls used :
Positive control
Negative control
12. a) How many times you have run this staining? :
12. b) Do you obtain the same results every time? :
12. c) What steps have you altered to try and optimize the use of this antibody? :
Document attachment :
Attaching images of your blot is strongly recommended and can greatly speed up our investigation of your problem. Make single zip file and attach.
 
 
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